DNA and RNA Extraction
The MetaSUB Consortium is currently testing various DNA and RNA extraction kits including:
- Omega BioTek
The consortium is also collaborating with the ABRF Metagenomics Research Group in developing a polyzyme mixture for DNA extraction.
Libraries will be prepared for sequencing using the QIAGEN QIAseq FX kits.
Samples will be sequencing using Illumina HiSeq next-generation shotgun sequencing technology.
In order to reduce any batch effects all consortium sample processing will occur at regional MetaSUB hubs:
- Americas: New York City
- Europe and Africa: Stockholm
- Asia and Oceania: Shanghai