DNA and RNA Extraction

The MetaSUB Consortium is currently testing various DNA and RNA extraction kits including:


  • MoBio
  • Promega
  • Zymo
  • Omega BioTek


The consortium is also collaborating with the ABRF Metagenomics Research Group in developing a polyzyme mixture for DNA extraction.


Library Preparation
Libraries will be prepared for sequencing using the QIAGEN QIAseq FX kits.


Samples will be sequencing using Illumina HiSeq next-generation shotgun sequencing technology.


In order to reduce any batch effects all consortium sample processing will occur at regional MetaSUB hubs:


  • Americas: New York City
  • Europe and Africa: Stockholm
  • Asia and Oceania: Shanghai