Satellite Sessions


The MetaSUB conference will have 3 satellite sessions on the University of Miami Miller School of Medicine campus on Friday, November 18, 2022, 10:00 am – 5:00 pm, prior to the start of the conference at the Fontainebleau hotel at 7:00 pm that day.  There are limited seats for each satellite session, so please register soon for the satellite session that you are interested in attending.

Field Demonstration of Wastewater Sample Collection and Processing

10:00 am - 12:00 pm ET

You are invited to view a field demonstration of wastewater sample collection, processing, and storage for metagenomic analysis, on the campus of the University of Miami Miller School of Medicine. This demonstration will include a discussion of disinfection safety measures, field data documentation of ambient conditions and water quality, and issues related to grab sample collection. Following the demonstration of waster sample collection in the field, participants will visit the Sylvester Biospecimen Shared Resource (BSSR), where the collected samples will be processed using electronegative filtration. Field protocols and laboratory SOPs will be shared.

Demonstration leader: Helena Solo-Gabriele, Ph.D., Professor of Chemical, Environmental, and Materials Engineering, University of Miami

Tour of the Sylvester Onco-Genomics Shared Resource and Sylvester Biospecimen Shared Resource at the University of Miami

1:00 pm - 3:00 pm ET

The Sylvester Onco-Genomics Shared Resource (OGSR) facilitates the genomics research of investigators from the Sylvester Comprehensive Cancer Center at the University of Miami and from the wider scientific community. The OGSR offers state-of-the-art next generation sequencing, single cell and spatial genomics, gene expression and molecular quantification, and sample processing for a wide array of research, including generation of metagenomics data for environmental and biomedical studies. The tour will provide an overview of the nucleic acid extraction and purification, library preparation, and sequencing techniques that the OGSR uses for environmental (e.g., wastewater) pathogen research and surveillance, and will also showcase new single cell and spatial genomics platforms.  The Sylvester Biospecimen Shared Resource (BSSR) provides biorepository services and expertise.  The BSSR facilitates translational research with specimen procurement and processing; quality control of specimens; secured specimen storage, retrieval, and distribution; and provision of relevant metadata.  The tour will provide an overview of the wastewater sample annotation, processing, storage and distribution services provided by the BSSR.  Tours will be interactive and both the shared resources staff will be happy to answer questions and share technical insights.

Tour leader: Sion Williams, Ph.D., Director of the Onco-Genomics Shared Resource, and Melinda Boone, Assistant Director of the Biospecimen Shared Resource, Sylvester Comprehensive Cancer Center, University of Miami

Workshop on Metagenomic Analysis with the MetaSUB Core Analysis Modular Pipeline (CAMP)

3:00 pm - 5:00 pm ET

This workshop will cover the tools needed to run and build cutting-edge, publishable metagenomic workflows using a diverse suite of cutting-edge metagenomic analysis tools. Analyzing microbial communities requires a series of complicated, algorithmically distinct steps, each of which can significantly influence results. We will show participants how to use the MetaSUB CAMP, an integrated suite of modular individually packaged tools that perform as a single consistently documented analytic workflow. CAMP modules can be linked together to carry out an entire workflow in a reproducible fashion, but each module has parameters that allow for maximal flexibility. CAMP is maintained by a globally distributed team, is easily updated, and is often updated with new metagenomics analysis approaches. In this hands-on workshop, participants will learn how to process their data using CAMP and how to develop new custom modules. Participants should bring a laptop capable of accessing a Linux virtual machine. We invite anyone with basic command line experience to attend this workshop and also -- if you want -- to become a CAMP developer.

Workshop leader: Braden Tierney, Ph.D., Postdoctoral Associate in Computational Biomedicine, Weill Cornell Medicine. Lauren Mak, Ph.D. Candidate, Weill Cornell Medicine